Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer

Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer

Blog Article

Summary: Esophageal squamous cell carcinoma (ESCC) is a common malignancy, characterized by a multistep pathogenic process regulated spatiotemporally within the esophageal epithelial microenvironment, including vessel normalization and immune infiltration.However, empirical evidence elucidating esophageal vascular remodeling and immune infiltration during ESCC tumorigenesis in situ is lacking.In this study, utilizing a mouse model recapitulating progressive human ESCC stages, we established a tissue gu502lv-bi7n8 clearing workflow for three-dimensional visualization and analysis of esophageal vessels and T cell distribution.Through this workflow, we delineated the spatial dynamics of vascular remodeling, CD3+ T cells, and characteristic T cell aggregates employing high-resolution light-sheet fluorescence microscopy across five ESCC pathogenic stages.Vessel remodeling might be coupled with T cell infiltration, and their interactions predominantly occurred ps5 price new jersey at the inflammatory stage.

These findings provided insights into research methodologies of esophageal cancer and spatiotemporal landscapes of vascular and T cell during ESCC initiation and progression.